Optimization of fermentation medium for extracellular lipase production from Aspergillus Niger using response surface methodology

11Citations
Citations of this article
45Readers
Mendeley users who have this article in their library.

Abstract

Lipase produced by Aspergillus Niger is widely used in various industries. In this study, extracellular lipase production from an industrial producing strain of A. Niger was improved by medium optimization. The secondary carbon source, nitrogen source, and lipid were found to be the three most influential factors for lipase production by single-factor experiments. According to the statistical approach, the optimum values of three most influential parameters were determined: 10.5 g/L corn starch, 35.4 g/L soybean meal, and 10.9 g/L soybean oil. Using this optimum medium, the best lipase activity was obtained at 2,171 U/mL, which was 16.4% higher than using the initial medium. All these results confirmed the validity of the model. Furthermore, results of the Box-Behnken Design and quadratic models analysis indicated that the carbon to nitrogen (C/N) ratio significantly influenced the enzyme production, which also suggested that more attention should be paid to the C/N ratio for the optimization of enzyme production.

Cite

CITATION STYLE

APA

Jia, J., Yang, X., Wu, Z., Zhang, Q., Lin, Z., Guo, H., … Wang, Y. (2015). Optimization of fermentation medium for extracellular lipase production from Aspergillus Niger using response surface methodology. BioMed Research International, 2015. https://doi.org/10.1155/2015/497462

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free