Comparative Evaluation of Bioefficiency and Photocatalytic Activity of Green Synthesized Bismuth Oxide Nanoparticles Using Three Different Leaf Extracts

Abstract

Nanotechnology has emerged as a promising method for wastewater recycling. In this line, the current study emphasizes the leaf-extract-mediated biosynthesis of bismuth oxide nanostructures (BiONPs) using three different plants, namely Coldenia procumbens Linn (Creeping Coldenia), Citrus limon (Lemon), and Murraya koenigii (Curry) through a greener approach and evaluates their biological properties as well as photocatalytic performance for the first time. As-synthesized BiONPs were physiochemically characterized using UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and high-resolution transmission electron microscopy (HRTEM) with energy dispersive X-ray analysis (EDAX). Using the well diffusion method, research on the antibacterial efficiency of BiONPs against human pathogenic Gram-positive bacteria, such as Staphylococcus aureus and Enterococcus faecalis, and Gram-negative bacteria, including Escherichia coli and Klebsiella pneumonia, revealed that Gram-negative bacteria exhibited relatively strong activity. The larvicidal activity assessed against Aedes aegypti and Aedes albopictus mosquito larvae reveals promising larvicidal activity with a minimal dosage of BiONPs with LC50 values of 5.53 and 19.24 ppm, respectively, after 24 h of exposure. The excellent photocatalytic activity of as-synthesized BiONPs was demonstrated through the photodegradation of malachite green (MG) and methylene blue (MB) dyes with respective degradation performance parameters of 70 and 90%. The biogenic synthetic approach reported here enables the scalable commercial synthesis of bismuth nanostructures for their widespread use in catalysis for wastewater treatment and environmental cleanup.