Biochemical effect of resveratrol on Lipids profile and hepatic oxidative stress in experimentally induced obesity in female rats

Abstract

This study was designed to evaluate the effect of prolonged intake of resveratrol on lipid metabolism, liver enzymes and L-malondialdehyde(L-MDA) in obesity-induced in female rats by feeding high fat diet. Ninety female albino rats were divided into six equal groups of 15 rats each. Group Ι: rats fed normal control diet all over the periods of experiment (for 12 weeks). Group II: rats fed the control normal diet and administered resveratrol orally (0.1 mg/kg b.wt., orally) for 6 weeks. Group III: rats received the control normal diet and received resveratrol once daily (0.5 mg/kg b.wt., orally) for 6 weeks. Group IV: rats fed high fat diet (HFD) and received no drugs all over the periods of experiment (for 12 weeks). Group V: rats received HFD and administered resveratrol (0.1 mg/kg b.wt./day, orally) for 6 weeks. Group VI: rats fed HFD and administered resveratrol (0.5 mg/kg b.wt./day, orally). Blood samples were collected from all rats at 2, 4 and 6 weeks from the onset of resveratrol administration for determination of serum total cholesterol(TC), Triacylglycerols (TAG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), very low density lipoprotein cholesterol (VLDL-c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and erythrocyte L-malondialdehyde (L-MDA).The obtained results showed, significant elevation of serum TC, TAG, LDL-c, VLDL-c, ALP,AST, ALT, L-MDA in obese rats as compared to rats fed normal control diet. While administration of resveratrol to HFD-fed rats tended to improve hepatic oxidative stress and dyslipidemia through improving total cholesterol and triacylglycerols and liver markers enzyme activities. These results suggest that, resveratrol is effective in improving the obesity and its associated many important complications such as diabetes mellitus and coronary heart disease and liver disease.