Anti-angiogenic effects of resveratrol in combination with 5-fluorouracil on B16 murine melanoma cells

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Abstract

5-Fluorouracil (5-FU) has been used as a chemotherapeutic drug for various types of cancer, although the development of resistance remains a major limitation for its use in clinical settings. In the present study, the anti-angiogenic effects of resveratrol and 5-FU either alone or in combination were examined in a B16 murine melanoma model. Co-treatment using resveratrol and 5-FU inhibited cell proliferation more efficiently compared with use of either drug alone and the antiproliferative effect coincided with changes in the expression levels of AMP-activated protein kinase (AMPK), cyclooxygenase-2, vasodilator-stimulated phosphoprotein (VASP) and vascular endothelial growth factor (VEGF). Furthermore, co-treatment with resveratrol and 5-FU reduced tumor growth compared with that in the control group and this growth-inhibitory effect was associated with changes in the expression levels of AMPK, VASP and VEGF. Immunohistochemical staining for angiogenesis demonstrated that co-treatment with resveratrol and 5-FU reduced the number of microvascular vessels compared with that in the control group. These results suggested that co-treatment with resveratrol and 5-FU suppressed cell growth and angiogenesis in B16 murine melanoma tumors.

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Lee, S. H. wa, Koo, B. S. eong, Park, S. Y. i., & Kim, Y. M. in. (2015). Anti-angiogenic effects of resveratrol in combination with 5-fluorouracil on B16 murine melanoma cells. Molecular Medicine Reports, 12(2), 2777–2783. https://doi.org/10.3892/mmr.2015.3675

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