Ca2+/calmodulin-dependent facilitation and inactivation of P/Q-type Ca2+ channels

Abstract

Trains of action potentials cause Ca2+-dependent facilitation and inactivation of presynaptic P/Q-type Ca2+ channels that can alter synaptic efficacy. A potential mechanism for these effects involves calmodulin, which associates in a Ca2+-dependent manner with the pore-forming α(1A) subunit. Here, we, report that Ca2+ and calmodulin dramatically enhance inactivation and facilitation of P/Q-type Ca2+ channels containing the auxiliary β(2a) subunit compared with their relatively small effects on channels with β(1b). Tetanic stimulation causes an initial enhancement followed by a gradual decline in P/Q-type Ca2+ currents over time. Recovery of Ca2+ currents from facilitation and inactivation is relatively slow (30 sec to 1 min). These effects are strongly inhibited by high intracellular BAPTA, replacement of extracellular Ca2+ with Ba2+, and a calmodulin inhibitor peptide. The Ca2+/calmodulin-dependent facilitation and inactivation of P/Q-type Ca2+ channels observed here are consistent with the behavior of presynaptic Ca2+ channels in neurons, revealing how dual feedback regulation of P/Q-type channels by Ca2+ and calmodulin could contribute to activity-dependent synaptic plasticity.