Masking effect of anti-androgens on androgenic activity in European river sediment unveiled by effect-directed analysis

110Citations
Citations of this article
78Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

This study shows that the androgen receptor agonistic potency is clearly concealed by the effects of androgen receptor antagonists in a total sediment extract, demonstrating that toxicity screening of total extracts is not enough to evaluate the full in vitro endocrine disrupting potential of a complex chemical mixture, as encountered in the environment. The anti-androgenic compounds were masking the activity of androgenic compounds in the extract with relatively high anti-androgenic potency, equivalent to 200 nmol flutamide equivalents/g dry weight. A two-step serial liquid chromatography fractionation of the extract successfully separated anti-androgenic compounds from androgenic compounds, resulting in a total androgenic potency of 3,820 pmol dihydrotestosterone equivalents/g dry weight. The fractionation simplified the chemical identification analysis of the original complex sample matrix. Seventeen chemical structures were tentatively identified. Polyaromatic hydrocarbons, a technical mixture of nonylphenol and dibutyl phthalate were identified to contribute to the anti-androgenic potency observed in the river sediment sample. With the GC/MS screening method applied here, no compounds with AR agonistic disrupting potencies could be identified. Seventy-one unidentified peaks, which represent potentially new endocrine disrupters, have been added to a database for future investigation.

Cite

CITATION STYLE

APA

Weiss, J. M., Hamers, T., Thomas, K. V., Van Der Linden, S., Leonards, P. E. G., & Lamoree, M. H. (2009). Masking effect of anti-androgens on androgenic activity in European river sediment unveiled by effect-directed analysis. In Analytical and Bioanalytical Chemistry (Vol. 394, pp. 1385–1397). https://doi.org/10.1007/s00216-009-2807-8

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free