Generation of surface-bound multicomponent protein gradients

Abstract

(Figure Presented) Spatial control of bioactive ligands is achieved by integrating microfluidics and protein engineering. The proteins of interest are mixed in a gradient generator and immobilized on artificial polypeptide scaffolds through the strong association of heterodimeric ZE/ZR leucine zipper pairs. Protein densities and gradient shapes are easily controlled and varied in this method. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.