Studies on catabolite repression in solid state fermentation for biosynthesis of fungal amylases

Abstract

Catabolite repression by glucose of the biosynthesis of alpha amylase and amyloglucosidase by Aspergillus niger CFTRI 1105 was studied in a solid state fermentation (SSF) and in submerged fermentation (SMF) systems and the results were compared. The addition of glucose did not enhance the production of alpha-amylase and amyloglucosidase in an earlier fermentation system. However, a drastic reduction in alpha-amylase production was observed in submerged fermentation by the addition of 5.0 mg ml-1 glucose and of amyloglucosidase production by 10 mg ml-1 glucose. Glucose concentrations above 50 mg ml-1 completely suppressed the production of both enzymes in the initial hours. In contrast, in the SSF system the repression was negligible, even when the glucose level was raised to 150 mg g-1 wheat bran for both alpha and amyloglucosidase synthesis.