Generation and Characterization of Patient-Derived Head and Neck, Oral, and Esophageal Cancer Organoids

64Citations
Citations of this article
86Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Esophageal cancers comprise adenocarcinoma and squamous cell carcinoma, two distinct histologic subtypes. Both are difficult to treat and among the deadliest human malignancies. We describe protocols to initiate, grow, passage, and characterize patient-derived organoids (PDO) of esophageal cancers, as well as squamous cell carcinomas of oral/head-and-neck and anal origin. Formed rapidly (<14 days) from a single-cell suspension embedded in basement membrane matrix, esophageal cancer PDO recapitulate the histology of the original tumors. Additionally, we provide guidelines for morphological analyses and drug testing coupled with functional assessment of cell response to conventional chemotherapeutics and other pharmacological agents in concert with emerging automated imaging platforms. Predicting drug sensitivity and potential therapy resistance mechanisms in a moderate-to-high throughput manner, esophageal cancer PDO are highly translatable in personalized medicine for customized esophageal cancer treatments. © 2020 by John Wiley & Sons, Inc. Basic Protocol 1: Generation of esophageal cancer PDO. Basic Protocol 2: Propagation and cryopreservation of esophageal cancer PDO. Basic Protocol 3: Imaged-based monitoring of organoid size and growth kinetics. Basic Protocol 4: Harvesting esophageal cancer PDO for histological analyses. Basic Protocol 5: PDO content analysis by flow cytometry. Basic Protocol 6: Evaluation of drug response with determination of the half-inhibitory concentration (IC50). Support Protocol: Production of RN in HEK293T cell conditioned medium.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Karakasheva, T. A., Kijima, T., Shimonosono, M., Maekawa, H., Sahu, V., Gabre, J. T., … Nakagawa, H. (2020). Generation and Characterization of Patient-Derived Head and Neck, Oral, and Esophageal Cancer Organoids. Current Protocols in Stem Cell Biology, 53(1). https://doi.org/10.1002/cpsc.109

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 30

77%

Researcher 7

18%

Lecturer / Post doc 2

5%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 15

43%

Medicine and Dentistry 10

29%

Agricultural and Biological Sciences 6

17%

Pharmacology, Toxicology and Pharmaceut... 4

11%

Save time finding and organizing research with Mendeley

Sign up for free