Transient expression using agroinfiltration to study polyadenylation in plants

Abstract

An important facet of the study of polyadenylation in plants entails the use of transient expression to characterize poly(A) sites and the subunits of the polyadenylation complex. In this report, a simple and adaptable approach to transient expression in Nicotiana benthamiana leaves is described. This approach has been used to evaluate protein-protein interactions amongst different plant polyadenylation factor subunits and to study the usage and behavior of individual poly(A) sites from plant genes.