Determination of Total Flavonoid Level and Antioxidant Activity of Ethyl Acetate Fraction of Mangkokan Leaf Extract (Nothopanax scutellarium [Burm.f] Merr.)

Abstract

​Mangkokan leaves (Nothopanax scutellarium (Burm.f) Merr.) have several properties, containing several chemicals that have bioactivity. It contains flavonoids and is a natural source of antioxidants. This study aims to determine the total flavonoid content and the potency of the ethyl acetate fraction in the leaves of the Mangkokan leaf as an antioxidant in counteracting free radicals. Identification of flavonoids was carried out with a reagent between magnesium powder and concentrated HCl, the orange color formed indicated the presence of flavonoids. Determination of the total flavonoid content of the ethyl acetate fraction of the Mangkokan leaf extract was carried out colorimetrically using a UV-Vis spectrophotometer. The antioxidant activity test of the ethyl acetate fraction of kukukan leaf extract was carried out using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The results showed that the total flavonoid content of the ethyl acetate fraction of the Mangkokan leaf extract was 25.402749 mgGAE/g extract and the free radical inhibition test with the DPPH method showed that the ethyl acetate fraction of the Mangkokan leaf extract had antioxidant activity with an IC50 value of 103.36 ppm. The ethyl acetate fraction of the Mangkokan leaf extract has activity as an antioxidant with a moderate category of antioxidant power so that it has the potential to be developed in the treatment of diseases caused by free radicals.