In vitro evaluation of isolated Staphylococcal-bacteriophage in killing methicillin-resistant Staphylococcus aureus

Abstract

The present study was carried out to isolate and identified the Staphylococcal-specific bacteriophage from swage water by using agar overlay method and to investigate the anti-Staphylococcal activity of Isolated Bacteriophage in-vitro. Two experiments were performed in this present study; the first one was isolation and identification of Methicillin-Resistant Staphylococcus aureus (MRSA) from post-traumatic bone infection. While the second experiment included study the infectivity of bacteriophage in-vitro to MRSA as well as effect of temperature and pH on the infectivity of phage was also studied. Results of antimicrobial susceptibility test of S. aureus isolates revealed that most of isolates were resistant to Methicillin, Cefoxitin and Pencillin G. Staphylococcus aureus phage was isolated from sewage samples by agar overlay method, the phage was characterized by clear, circular plaques ranged between 2-3 mm in diameter. TTA method for determined the Phage MIC appears to be both reliable, and reproducible. This method is laborious and time consuming, and the proposed MIC method appears to be an interesting alternative.