Drought stress induces a biphasic NO accumulation in Arabidopsis thaliana

Abstract

We have recently reported the proteomic signature of the early (≤30 min) drought stress responses in Arabidopsis thaliana suspension cells challenged with PEG. We found an over-representation in the gene ontology categories "Ribosome" and "Oxidative stress along with an increased abundance of late embryogenesis abundant (LEA) and early response to dehydration (ERD) proteins. Since nitric oxide (NO) plays a pivotal role in plant responses to drought stress and induces LEA and DREB proteins, here we monitored the levels of NO in Arabidopsis cell suspensions and leaf disks challenged with PEG, and performed comparative analyses of the proteomics and transcriptomics data in public domain to search for a common set of early drought and NO responsive proteins. We show that under drought-stress, NO shows a biphasic time course, much like in response to ozone stress and that among the early drought and NO responsive proteins, the categories "DNA binding", "Nucleotide binding" and "Transcription regulator activity" are enriched. Taken together, present study suggests that in Arabidopsis the changing NO levels may play a critical role in early drought responsive processes and notably in the transcriptional and translational reprograming observed under drought stress.