Activity-guided isolation and identification of antioxidant components from ethanolic extract of Peltiphyllum peltatum (Torr.) Engl.

Abstract

Activity-directed fractionation and isolation procedures were used to identify the antioxidant components of Peltiphyllum peltatum. Fresh leaves were extracted with ethanol and subsequently fractionated into light petroleum, chloroform, ethyl acetate, n-butanol, and water fractions. The ethyl acetate fraction, which showed the strongest antioxidant activity, was subjected to Sephadex LH-20 chromatography, followed by repetitive preparative TLC, to afford the major antioxidant principle, quercitrin, together with other antioxidants (isoquercitrin, catechin and gallate) as minor principles. The identities of the isolated compounds were established by spectroscopic methods (1D and 2D NMR and MS) and by direct comparison with authentic samples and literature values. Quercitrin displayed a potent protective effect of DNA from UV generated hydroxyl radical damage.