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Using solid-state 13C NMR spectroscopy to study the molecular organisation of primary plant cell walls.

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Abstract

Studies of the mobilities of polysaccharides or parts of polysaccharides in a cell-wall preparation may give clues about the molecular interactions among the polysaccharides in the cell wall and the relative locations of polysaccharides within the cell wall. A number of solid-state (13)C NMR techniques have been developed that can be used to investigate different types of polysaccharide mobilities: rigid, semi-rigid, mobile, and highly mobile. In this chapter, techniques are described for obtaining spectra from primary cell-wall preparations using CP/MAS, proton-rotating frame, proton spin-spin, spin-echo relaxation spectra, and single-pulse excitation. We also describe how proton spin relaxation editing can be used to obtain subspectra for cell-wall polysaccharides of different mobilities.

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Bootten, T. J., Harris, P. J., Melton, L. D., & Newman, R. H. (2011). Using solid-state 13C NMR spectroscopy to study the molecular organisation of primary plant cell walls. Methods in Molecular Biology (Clifton, N.J.), 715, 179–196. https://doi.org/10.1007/978-1-61779-008-9_13

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