Selection of Root-Knot Nematod Resistance in Inbred Tomato Lines Using CAPS Molecular Markers

Abstract

Tomato root-knot nematode, is one of the most important disease agents in tomatoes (Solanum lycopersicum).  A practical and effective method for controlling this disease is by means of tomato genotypes with resistance genes against this disease.  Tomato plants are offered resistance to root-knot nematode by Mi gene. Phenotypically observing genetic lines in terrestrial conditions is difficult and time consuming due to concerns about the transfer of this agent to other plants and their stability. By using the molecular markers related to resistance genes, this problem can be avoided and the selection efficiency can be increased. In this project, 34 inbred lines were tested using 1 CAPS marker associated with Mi gene, resistant and susceptible genotypes were identified.  In the study, 34 genotypes were not found to be homozygous resistant (MI / MI) genotypes whereas 7 genotypes were found to be heterozygous resistant (MI / mi) and 27 genotypes (mi / mi)homozygous susceptible. As a result of this study, root-knot nematode is the basis for developing resistant varieties.