Expression of the Arabidopsis abi1-1 mutant allele inhibits proteinase inhibitor wound-induction in tomato

Abstract

Abscisic acid (ABA) is an essential component in the wound signalling cascade. Increased levels of endogenous ABA were observed after wounding and shown to be a requisite for wound-induced expression of the proteinase inhibitor II genes. We have taken advantage of the dominant character of the Arabidopsis abi1-1 mutation, to investigate whether ABI1 has a function in ABA signalling in response to wounding. Transgenic tomato plants carrying copies of either the wild-type ABI1 or the mutant abi1-1 alleles were obtained and assayed for wound-induction of the pin2 or LAP genes. While normal levels of gene induction were observed in the transgenic ABI1 plants, the abi1-1 transformants displayed a severe wilty phenotype and reduced seed dormancy. Expression of the abi1-1 dominant mutation blocked accumulation of the drought-induced TAS14 and LE25 mRNAs in response to ABA, as well as ABA- and wound-induced expression of the defense-associated pin2 and LAP transcripts. MeJA-induction of the pin2 and LAP mRNAs, on the contrary, was not affected in the abi1-1 transformants. These results indicate that abi1-1 inhibits wound-induced expression of the pin2 and LAP transcripts by blocking ABA-induction of these genes. This implicates ABI1 in wound-signalling and suggests that a common early ABA signalling pathway may function in the responses to wounding and water stress.