Detection of extended spectrum beta-lactamases producing Enterobacteriaceae in feces

Abstract

A study was made of 366 feces for detection of extended spectrum beta-lactamases producing Enterobacteriaceae from feces. The selective agar was used for modified drigalski agar (Eiken Chemical Co., LTD) with 2 micrograms/ml cefotaxime (ESBL screen agar). 92 strains of Enterobacteriaceae, 41 Escherichia coli, 15 Citrobacter freundii, 13 Enterobacter cloacae, 11 Klebsiella pneumoniae, and other 12, were isolated from ESBL screen agar. And, R-plasmid that were selected by 2 micrograms/ml cefotaxime were transferred by conjugation from two of the 92 strains. These strain were E. coli TH9809927 and Proteus mirabilis TH9808262 that were amplified by "Toho-1 type" primer. The clude enzyme from two strains (donor) and transconjugants were especially hydrolysed cepharoridine and cefotaxime. Accordingly, two strains (0.5%) were detected as ESBL producers. We think that the result of our survey suggests the increase of ESBLs producing bacterial infection in Japan, and believe that there is a trend of infection of its by surveilance of the feces.