Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system

Abstract

A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/μ g) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50°C. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recuperation (99.8%) of enzymatic activity in the PEG phase.