Isolation of Bacteria Contaminants from Tetra Packed Yoghurt

Abstract

This work was designed to isolate the microorganisms associated with the spoilage of yogurt products using standard microbiological and biochemical procedure. Six different brands of tetra packed yoghurt which had over stayed and the shelf-life deteriorated were purchased from the production company, market stores that were unable to sell out their yoghurt prior to the expiry date, and from hawkers in Abakaliki metropolis. Two samples of each brand were used and the brand were designated A, B, C, D, E, F, giving a total of 12 yoghurt samples. Bergey's manual was used for bacteria identification. The results reveales the presence of Klebsiella pneumonia, Citrobacter sp, Pseudomonas sp. Escherichia coli. Proteus sp and Vibrio sp. in the samples. The isolation of Citrobacter sp, klebsiella pneumonia, Escherichia coli. Pseudomonas sp, Proteus sp and Vibrio sp is a clear indication of a deplorable state of poor hygienic and sanitary practices employed in the processing and packaging of the yoghurt product.