Diversity, Composition, and Ecological Function of Endophytic Fungal Communities Associated with Erigeron breviscapus in China

Abstract

Endophytic fungi (EF) play crucial roles in accelerating the accumulation of medicinal components and regulating the survival and reproduction of medicinal plants. Erigeron breviscapus is a well-known traditional Chinese medicinal plant with effective therapeutic effects and a wide application, but little is known about the diversity, community structure, and ecological roles of EF in this plant. Here, the EF communities associated with different tissues of E. breviscapus at two seasons were studied by high-throughput sequencing methods. Furthermore, FUNGuild was performed to predict the ecological functions of the fungi. Didymella was found to be the most dominant genus across all four tissues, followed by Plectosphaerella, Filobasidium, Cystofilobasidium, and Cladosporium. Notably, the dominant and unique genera and biomarkers of four tissues were different. Interestingly, it was found that the roots had the highest fungal richness and diversity in summer. Moreover, both PCoA plots and PERMANOVA analyses indicated that the tissue and season were main factors contributing to the differences in the fungal communities of E. breviscapus. FUNGuild prediction revealed that pathotroph–saprotroph fungi and undefined taxa accounted for a high proportion in the EF of E. breviscapus. We also found some valuable endophytes that encouraged deeper investigation. These findings provide a theoretical reference for the further development and utilization of EF resources in E. breviscapus.