3 Yeast Transformation

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Abstract

The efficient transformation of yeast has allowed much progress in the field of yeast molecular biology. For techniques such as two-hybrid analysis and yeast genome modification, transformation is an essential step. We have presented a protocol for rapid transformation that is useful for putting plasmids into yeast strains. An efficient transformation protocol is also presented, which can be used for library screening and genome modification. In addition, we have developed two microtitre plate protocols, which can be used and adapted with current high throughput methodology. Finally, we have developed a protocol for the production of frozen competent yeast cells. This protocol allows large quantities of yeast cells that are competent for transformation to be prepared and frozen. Highly efficient transformation can then be performed at a moment's notice without the required pre-culture and re-growth steps. © 2007 Elsevier Ltd. All rights reserved.

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APA

Truong, D., & Gietz, R. D. (2007). 3 Yeast Transformation. Methods in Microbiology. https://doi.org/10.1016/S0580-9517(06)36003-5

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