Isolation and identification of lactic acid bacteria in hive of Apis dorsata from semi-arid tropical climate in Benu village, East Nusa Tenggara

Abstract

Lactic Acid Bacteria (LAB) have been isolated from the hive of Giant Honey Bee (Apis dorsata) origin from tropical climate. Information about the diversity of LAB from the hive of A. dorsata from Benu Village has not been found yet. The information is important as a preliminary study of the potential of biological resources for food functional development in the future. Therefore, it is needed to research on diversity of LAB that isolated from the hive of A. dorsata from the village of Benu as an area of semi-arid tropical climate. The purpose of this study is determine the diversity of LAB isolated from the hive of A. dorsata from Benu Village. This research is important because it provides preliminary information about the diversity of LAB species from the hive of A. dorsata from Benu Village as a representation of semi-arid tropical climate. Profile of Denaturing Gradient Gel Electrophoresis showed the presence of seven LAB isolates from the hive of A. dorsata in Benu Village. This is an early indication that hive of A. dorsata from semi-arid tropic climate has the potential as a biological resources of LAB that important for food funtional development in the future.