Nucleoside transport in human colonic epithelial cell lines: Evidence for two Na+-independent transport systems in T84 and Caco-2 cells1

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Abstract

RT-PCR of RNA isolated from monolayers of the human colonic epithelial cell lines T84 and Caco-2 demonstrated the presence of mRNA for the two cloned Na+-independent equilibrative nucleoside transporters, ENT1 and ENT2, but not for the cloned Na+-dependent concentrative nucleoside transporters, CNT1 and CNT2. Uptake of [3H]uridine by cell monolayers in balanced Na+-containing and Na+-free media confirmed the presence of only Na+-independent nucleoside transport mechanisms. This uptake was decreased by 70-75% in the presence of 1 μM nitrobenzylthioinosine, a concentration that completely inhibits ENT1, and was completely blocked by the addition of 10 μM dipyridamole, a concentration that inhibits both ENT1 and ENT2. These findings indicate the presence in T84 and Caco-2 cells of two functional Na+-independent equilibrative nucleoside transporters, ENT1 and ENT2. Copyright (C) 1999 Elsevier Science B.V.

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Ward, J. L., & Tse, C. M. (1999). Nucleoside transport in human colonic epithelial cell lines: Evidence for two Na+-independent transport systems in T84 and Caco-2 cells1. Biochimica et Biophysica Acta - Biomembranes, 1419(1), 15–22. https://doi.org/10.1016/S0005-2736(99)00045-0

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