In Vitro Biocompatibility Assessment of a Novel Membrane Containing Magnesium-Chitosan/Carboxymethyl Cellulose and Alginate Intended for Bone Tissue Regeneration

Abstract

Bone tissue engineering (BTE) is an emerging interdisciplinary field that aims to develop new strategies and materials for repairing, regenerating, or replacing damaged bone tissues. This field combines engineering, biology, and medicine principles to create functional bone tissues in the laboratory and in vivo. The main goal of BTE is to create biological substitutes that mimic the structure, function, and properties of natural bone tissue, thereby promoting the regeneration of bone defects caused by trauma, disease, or aging. In this study, we developed a biocomposite membrane using magnesium-chitosan, carboxymethyl cellulose, and alginate through a simple cast drying method. The biocompatibility of the membrane was evaluated using human osteoblastic cells, and it was found to be nontoxic to these cells. Both metabolic activity measurements (24 and 48 hours) and the lactate dehydrogenase release assay (72 hours) indicated that the membrane was biocompatible and did not exert significant toxic effects. These results suggest that the developed biocomposite membrane has the potential to be used as a safe and effective biomaterial for various biomedical applications, such as BTE, wound healing, and drug delivery. Further studies are warranted to explore the full potential of this membrane and its performance in different biological environments.