An Approach for Micropropagation of Blueberry (<i>Vaccinium corymbosum</i> L.) Plants Mediated by Temporary Immersion Bioreactors (TIBs)

  • Arencibia A
  • Vergara C
  • Quiroz K
  • et al.
N/ACitations
Citations of this article
44Readers
Mendeley users who have this article in their library.

Abstract

A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO 2 enrichment, sucrose-reduced medium, and light intensity of 60 M m −2 ·s −1 . Principal component analysis showed that component 1 (C1) grouped 64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adapt-ability and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a photomixotrophic stage in the vitroplantlets cultured in TIBs.

Cite

CITATION STYLE

APA

Arencibia, A. D., Vergara, C., Quiroz, K., Carrasco, B., Bravo, C., & Garcia-Gonzales, R. (2013). An Approach for Micropropagation of Blueberry (<i>Vaccinium corymbosum</i> L.) Plants Mediated by Temporary Immersion Bioreactors (TIBs). American Journal of Plant Sciences, 04(05), 1022–1028. https://doi.org/10.4236/ajps.2013.45126

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free