Speeding up enzyme discovery and engineering with ultrahigh-throughput methods

Citations of this article
Mendeley users who have this article in their library.


Exploring the sequence space of enzyme catalysts is ultimately a numbers game. Ultrahigh-throughput screening methods for rapid analysis of millions of variants are therefore increasingly important for investigating sequence-function relationships, searching large metagenomic libraries for interesting activities, and accelerating enzyme evolution in the laboratory. Recent applications of such technologies are reviewed here, with a particular focus on the practical benefits of droplet-based microfluidics for the directed evolution of natural and artificial enzymes. Broader implementation of such rapid, cost-effective screening technologies is likely to redefine the way enzymes are studied and engineered for academic and industrial purposes.




Bunzel, H. A., Garrabou, X., Pott, M., & Hilvert, D. (2018, February 1). Speeding up enzyme discovery and engineering with ultrahigh-throughput methods. Current Opinion in Structural Biology. Elsevier Ltd. https://doi.org/10.1016/j.sbi.2017.12.010

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free