Copy number variation of agouti signaling protein (ASIP) fragment and its relationship with coat color in Indonesian goat breeds

Abstract

Background: The coat color is important for the production of wool, for heat tolerance in hot climates, cultural value and for the identification of the breed. Goat has very important roles in the agricultural economies of Indonesia. The goat is not only to produce meat, milk, wool and compost, but also for cultural and religious events. Batak tribe in North Sumatra has a unique tradition of customary celebration by using goat. Goat that has completely white coat should be used, so that the white coat has economic value much higher than other colors. Materials and Methods: This study used blood from each individual. Four subpopulations of domestic goat breeds in Indonesia, namely Samosir goat, Kacang goat, Gembrong goat and Peranakan Etawah goat. The agouti signaling protein (ASIP) gene fragments amplified and sequenced. The length of the ASIP gene amplification products was 610 bp for every individual and analyzed to detect variations on every Indonesian local goat. Results: From all samples of Indonesian goats, there is only one difference on Samosir goat 1 c.158 G-T. This polymorphism only foundin Samosir goat 1 while the other Indonesian local goats and other organisms were the similar. This variation would change to the order of amino acids in goat Samosir 1. Conclusion: There is no variation in the ASIP fragment of Indonesian goats that can be used to investigate the relationship between exon 4 of ASIP and coat color expression of Indonesia local goats. The exon 4 of the ASIP cannot be made target to investigate different coat color in some Indonesian goat breeds.