STED and STORM superresolution imaging of primary cilia

Citations of this article
Mendeley users who have this article in their library.
Get full text


The characteristic lengths of molecular arrangement in primary cilia are below the diffraction limit of light, challenging structural and functional studies of ciliary proteins. Superresolution microscopy can reach up to a 20 nm resolution, significantly improving the ability to map molecules in primary cilia. Here we describe detailed experimental procedure of STED microscopy imaging and dSTORM imaging, two of the most powerful superresolution imaging techniques. Specifically, we emphasize the use of these two methods on imaging proteins in primary cilia.




Tony Yang, T. T., Chong, W. M., & Liao, J. C. (2016). STED and STORM superresolution imaging of primary cilia. In Methods in Molecular Biology (Vol. 1454, pp. 169–192). Humana Press Inc.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free