GENOTYPIC VARIABILITY OF PEANUT LINES IN RESPONSE TO WATER STRESS, BASED ON BIOCHEMICAL DESCRIPTORS

  • ALVES G
  • PEREIRA J
  • LUZ L
  • et al.
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Abstract

ABSTRACT Seven biochemical descriptors were used to estimate the genotypic variability of peanut in response to moderate water stress. Six genotypes, constituted by four lines and two cultivars, were grown in pots, each containing two plants. At 15 days after emergence (DAE), the treatment differentiation was carried out: Control-plants maintained with daily watering, and Stress-plants submitted to water stress by complete suspension of watering for 15 days. The experimental design was completely randomized with factorial scheme 6 x 2 (genotype x water treatments), with five replications. The biochemical variables evaluated were: catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), free proline, total carbohydrates, soluble proteins, and amino acids. Results obtained by biochemical analysis and estimation of genotypic variability indicated that proline is the most appropriate descriptor for selecting genotypes tolerant to water stress, which led to identification of L81V and L108V as promising lines for drought tolerance breeding program.RESUMO Sete descritores bioquímicos foram utilizados para estimar a variabilidade genotípica de linhagens de amendoim submetidas a estresse hídrico moderado. Seis genótipos, sendo quatro linhagens e duas cultivares, foram cultivados em vasos, cada um contendo duas plantas e mantidas em casa de vegetação. Aos 15 dias após a emergência, procedeu-se a diferenciação dos tratamentos: Controle, as plantas mantidas com rega diariamente; Estresse, as plantas submetidas à suspensão total de rega durante 15 dias. O delineamento experimental foi inteiramente casualizado, com esquema fatorial 6 x 2 (genótipos x tratamentos hídricos), com cinco repetições. As variáveis bioquímicas avaliadas foram: catalase (CAT), ascorbato peroxidase (APX), guaiacol peroxidase (GPX), prolina livre, carboidratos totais, proteínas solúveis e aminoácidos. Baseado nos resultados das análises bioquímicas e de estimativa da variabilidade genotípica, a prolina demonstrou ser o descritor mais adequado para seleção de genótipos tolerantes ao estresse hídrico, contribuindo para indicar as linhagens L81V e L108V como mais promissoras, para um programa de melhoramento visando tolerância à seca.

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APA

ALVES, G. M. R., PEREIRA, J. W. D. L., LUZ, L. N. D., LIMA, L. M. D., & SANTOS, R. C. D. (2016). GENOTYPIC VARIABILITY OF PEANUT LINES IN RESPONSE TO WATER STRESS, BASED ON BIOCHEMICAL DESCRIPTORS. Revista Caatinga, 29(3), 528–536. https://doi.org/10.1590/1983-21252016v29n302rc

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