A rapid and simple protocol for the isolation of bacteriophages from coastal organisms

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Abstract

This work details a protocol for recovering bacteriophages from intertidal sessile mussels and testing their lytic activity against pathogenic bacteria. Although bacteriophages were highly abundant in coastal filter-feeding organisms, they were not detectable in the surrounding water column. This difference reflects the high filtering rate of the mussels, which capture and concentrate high amounts of bacteria, generating an ideal environment for bacteriophages. We validated the protocol providing a mean concentration of 4E + 04 PFU mL-1 lytic bacteriophages for the fish-pathogen bacterium Vibrio ordalii. We suggest that this method has particular utility for the recovery of bacteriophages for use as natural antimicrobial agents in aquaculture.

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Echeverría-Vega, A., Morales-Vicencio, P., Saez-Saavedra, C., Gordillo-Fuenzalida, F., & Araya, R. (2019). A rapid and simple protocol for the isolation of bacteriophages from coastal organisms. MethodsX, 6, 2614–2619. https://doi.org/10.1016/j.mex.2019.11.003

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