Single-cell TCRseq: Paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq

74Citations
Citations of this article
257Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Accurate characterization of the repertoire of the T-cell receptor (TCR) alpha and beta chains is critical to understanding adaptive immunity. Such characterization has many applications across such fields as vaccine development and response, clone-tracking in cancer, and immunotherapy. Here we present a new methodology called single-cell TCRseq (scTCRseq) for the identification and assembly of full-length rearranged V(D)J T-cell receptor sequences from paired-end single-cell RNA sequencing reads. The method allows accurate identification of the V(D)J rearrangements for each individual T-cell and has the novel ability to recover paired alpha and beta segments. Source code is available at https://github.com/ElementoLab/scTCRseq.

Cite

CITATION STYLE

APA

Redmond, D., Poran, A., & Elemento, O. (2016). Single-cell TCRseq: Paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq. Genome Medicine, 8(1), 1. https://doi.org/10.1186/s13073-016-0335-7

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free