Achieving efficient cardiac gene transfer in a large animal model has proven to be technically challenging. Prior strategies have employed cardio-pulmonary bypass or dual catheterization with the aid of vasodilators to deliver vectors, such as adenovirus, adeno-associated virus (AAV) or plasmid DNA. While single-stranded AAV vectors have shown the greatest promise, they suffer from delayed expression, which might be circumvented by using self-complementary vectors. We have recently optimized a cardiac gene transfer protocol in the canine using a percutaneous transendocardial injection catheter to deliver an AAV vector under fluoroscopic guidance. Percutaneous transendocardial injection of self-complementary AAV (scAAV)-6 is a safe, effective method for achieving efficient cardiac gene transfer to approximately 60% of the myocardium.
CITATION STYLE
Bish, L. T., Sleeper, M. M., & Sweeney, H. L. (2011). Percutaneous Transendocardial Delivery of Self-Complementary Adeno-Associated Virus 6 in the Canine. In Methods in Molecular Biology (Vol. 709, pp. 369–378). Humana Press Inc. https://doi.org/10.1007/978-1-61737-982-6_24
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