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Identification of brassinosteroid target genes by chromatin immunoprecipitation followed by high-throughput sequencing (Chip-seq) and RNA-sequencing

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Abstract

Brassinosteroids (BRs) play important roles in many growth and developmental processes. BRs signal to regulate BR-INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1) transcription factors (TFs), which, in turn, regulate several hundreds of transcription factors (termed BES1/BZR1-targeted TFs or BTFs) and thousands of genes to mediate various BR responses. Chromatin Immunoprecipitation followed by high-throughput sequencing (ChIP-seq) with BES1/BZR1 and BTFs is an important approach to identify BR target genes. In combination with RNA-sequencing experiments, these genomic methods have become powerful tools to detect BR target genes and reveal transcriptional networks underlying BR-regulated processes.

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Nolan, T., Liu, S., Guo, H., Li, L., Schnable, P., & Yin, Y. (2017). Identification of brassinosteroid target genes by chromatin immunoprecipitation followed by high-throughput sequencing (Chip-seq) and RNA-sequencing. In Methods in Molecular Biology (Vol. 1564, pp. 63–79). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6813-8_7

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