Using blot overlay techniques we have investigated the interaction of vinculin with α-actinin. We show that an α-actinin binding site is located in the 90 kDa vinculin head and confirm a vinculin binding site in the C-terminal rod of α-actinin, as recently reported by McGregor et al. [(1994) Biochem. J. 310, 225-233]. The isolated vinculin head binds much more strongly to α-actinin than intact vinculin. Using a proteolytic 81 kDa head fragment, we show that vinculin residues 1-107 are required for α-actinin binding. Antibodies directed against vinculin residues 808-850 inhibit the vinculin-α-actinin binding, suggesting that this sequence is directly involved in, or topographically related to, the α-actinin binding site. © 1994.
Kroemker, M., Rüdiger, A. H., Jockusch, B. M., & Rüdiger, M. (1994). Intramolecular interactions in vinculin control α-actinin binding to the vinculin head. FEBS Letters, 355(3), 259–262. https://doi.org/10.1016/0014-5793(94)01216-4