Lunatic Fringe (l-Fng) is one of the vertebrate homologues of Drosophila Fringe, which interacts with the Notch signal pathway and regulates activation of the Notch ligands, Delta and Serrate. To elucidate the roles of l-Fng in vertebrate neurogenesis, we transfected chick l-Fng (C-l-Fng) to chick neural tube using the in ovo electroporation technique and examined the subsequent changes in expression of Notch-related genes. We observed downregulation of C-Serrate-1 by ectopic C-l-Fng expression which implied that C-l-Fng acts on the vertebrate Notch pathway to regulate the expression of its ligand.
Sakamoto, K., Nakamura, H., Takagi, M., Takeda, S., & Katsube, K. ichi. (1998). Ectopic expression of lunatic Fringe leads to downregulation of Serrate- 1 in the developing chick neural tube; analysis using in ovo electroporation transfection technique. FEBS Letters, 426(3), 337–341. https://doi.org/10.1016/S0014-5793(98)00369-X