Abstract
A chemically inducible acetamidase promoter-sigF fusion gene was integrated into the chromosome of Mycobacterium bovis BCG. Two-dimensional protein gel analysis permitted the identification of a number of protein spots whose expression was SigF related. One spot upregulated by inappropriate induction of sigF expression corresponded to the 16-kDa antigen alpha-crystallin.
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CITATION STYLE
Manabe, Y. C., Chen, J. M., Ko, C. G., Chen, P., & Bishai, W. R. (1999). Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue. Journal of Bacteriology, 181(24), 7629–7633. https://doi.org/10.1128/jb.181.24.7629-7633.1999
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