Co-selection of cognate antibody-antigen pairs by selectively-infective phages

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Abstract

We have developed a chloramphenicol resistant derivative of fd phage with which cognate pairs of antibodies and antigens can be selected. The phage genome encodes a fusion of single-chain antibody to the C-terminal domain of gIIIp, rendering the phage non-infective. The antigen fused to the N-terminal domains of gIIIp is encoded in the same phage genome. Antigen and antibody fusion interact with each other in the periplasm of the phage-producing cell, restoring infectivity. This system has a very low background and will allow simultaneous randomisation of antibody and antigen. © 1995.

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Krebber, C., Spada, S., Desplancq, D., & Plückthun, A. (1995). Co-selection of cognate antibody-antigen pairs by selectively-infective phages. FEBS Letters, 377(2), 227–231. https://doi.org/10.1016/0014-5793(95)01348-2

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