In vitro inhibitory and pro-apoptotic effect of Stellera Chamaejasme L Extract on human lung cancer cell line NCI-H157

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Abstract

OBJECTIVE: To investigate the inhibitory and pro-apoptotic effect of Stellera Chamaejasme L extract (ESC) in vitro. METHODS: ESC was first extracted with ethanol, and then washed using a polyamide column with 60% ethanol. ESC was then decompressively recycled and vacuum dried at room temperature to obtain active fractions. Subsequently, the cytotoxic and apoptotic effects of ESC on NCI-H157 human lung cancer cells were determined. RESULTS: The results showed that ESC was rich in isomers of Chamaejasminor, neochamaejasmine and Sikokianin. ESC had significant cytotoxicity against NCI-H157 cells, with an IC50 of approximately 18.50 μg·mL-1. ESC caused significant increase in total apoptotic rate, the activity of caspase 3 and 8, and Fas protein expression (P<0.05). CONCLUSION: The inhibitory effect of ESC on NCI-H157 tumor cells might partly be attributed to its apoptotic induction through activation of the Fas death receptor pathway. © 2012 JTCM. All rights reserved.

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Liu, X., Li, Y., Yang, Q., Chen, Y., Weng, X., Wang, Y., … Zhu, X. (2012). In vitro inhibitory and pro-apoptotic effect of Stellera Chamaejasme L Extract on human lung cancer cell line NCI-H157. Journal of Traditional Chinese Medicine, 32(3), 404–410. https://doi.org/10.1016/s0254-6272(13)60046-1

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