The 16S rRNA gene is widely used for taxonomic profiling of microbial ecosystems; and recent advances in sequencing chemistry have allowed extremely large numbers of sequences to be generated from minimal amounts of biological samples. Analysis speed and resolution of data to species-level taxa are two important factors in large-scale explorations of complex microbiomes using 16S sequencing. We present here new software, Phylogenetic Tools for Analysis of Species-level Taxa (PhyloToAST), that completely integrates with the QIIME pipeline to improve analysis speed, reduce primer bias (requiring two sequencing primers), enhance species-level analysis, and add new visualization tools. The code is free and open source, and can be accessed at http://phylotoast.org.
CITATION STYLE
Dabdoub, S. M., Fellows, M. L., Paropkari, A. D., Mason, M. R., Huja, S. S., Tsigarida, A. A., & Kumar, P. S. (2016). PhyloToAST: Bioinformatics tools for species-level analysis and visualization of complex microbial datasets. Scientific Reports, 6. https://doi.org/10.1038/srep29123
Mendeley helps you to discover research relevant for your work.