Low density lipoprotein-oxidation inhibitory phytochemicals from the fruits of rhus parviflora

Abstract

Fruits of Rhus parviflora were extracted with 80% aqueous methanol (MeOH), and the concentrated extract was partitioned using ethyl acetate (EtOAc), n-butanol (n-BuOH), and H 2 O, successively. Purification of EtOAc fraction led to isolation of fifteen polyphenols of which structures were identified by spectroscopic methods including 2D-NMR. Most compounds apart from compound 10 inhibited low density lipoproteinoxidation within IC 50 value of 10 µM. Among compounds, taxifolin (2), quercetin 3-O-α-L-rhamnopyranoside (13), agathisflavone (5) sulfuretin (4), and aureusidin (3) showed IC 50 values 0.9, 0.8, 5.8, 2.9, and 2.4 µM which were of highly significant in comparison positive control butylated hydroxytoluene with IC 50 value of 2.1 µM. The results indicate fruits of R. parviflora as a source of antihypercholesterolemic compounds.