We have expressed green fluorescent protein (GFP) from A. victoria in the secretory pathway of HeLa cells by fusing it to the C-terminus of a secretory protein, chromogranin B. Under normal culture conditions at 37°C maturation of GFP to the fluorescent form was not detectable. However, fluorescent GFP was observed when biosynthetic protein transport was arrested at the intermediate compartment or the trans-Golgi network by temperature blocks (15°C and 20°C, respectively). Reversal of the temperature blocks allowed the visualization of secretion of fluorescent GFP and offers the possibility to analyse transport in the secretory pathway in living cells. © 1995.
Kaether, C., & Gerdes, H. H. (1995). Visualization of protein transport along the secretory pathway using green fluorescent protein. FEBS Letters, 369(2–3), 267–271. https://doi.org/10.1016/0014-5793(95)00765-2