A highly sensitive and selective cathodic adsorptive stripping voltammetric method for determination of rutin is presented. The method relies on the accumulation of a Cu(II)-rutin complex at a hanging mercury drop electrode (HMDE), followed by its reduction during a differential pulse voltammetric scan. The electrochemical behavior of the Cu(II)-rutin complex at HMDE was investigated by cyclic voltammetry. Results show that the electrode process is adsorption-controlled and gradually becomes less reversible at high scan rates where peak separation grows. Under the optimized conditions (phosphate buffer pH 6, -1.000V accumulation potential, 180s accumulation time, 70mV pulse amplitude, 50mVs -1 scan rate and 1.6×10 -6M Cu(II) concentration), the reduction peak current (I pc) of the Cu(II)-rutin complex is linear (I pc (nA)=10.070+1.9×10 8 [Rutina]) to rutin concentration in the range from 2.0×10 -7 to 1.4×10 -6M, with a correlation coefficient of 0.999. The detection and quantification limits obtained were 7.0×10 -9M and 2.2×10 -8M, respectively. The method was successfully applied to the determination of rutin in soybean cultivars, with recoveries of 94-105%. © 2011 Elsevier Inc.
da Silva, J. G., e Silva, M. R. L., de Oliveira, A. C., SouzaDe, J. R., Vaz, C. M. P., & de Castro, C. S. P. (2012). Cathodic adsorptive stripping voltammetric determination of rutin in soybean cultivars. Journal of Food Composition and Analysis, 25(1), 1–8. https://doi.org/10.1016/j.jfca.2011.04.013