Cohesin is a conserved, ring-shaped protein complex that topologically embraces DNA. Its central role in genome organization includes functions in sister chromatid cohesion, DNA repair, and transcriptional regulation. Cohesin loading onto chromosomes requires the Scc2-Scc4 cohesin loader, whose presence on chromatin in budding yeast depends on the RSC chromatin remodeling complex. Here we reveal a dual role of RSC in cohesin loading. RSC acts as a chromatin receptor that recruits Scc2-Scc4 by a direct protein interaction independent of chromatin remodeling. In addition, chromatin remodeling is required to generate a nucleosome-free region that is the substrate for cohesin loading. An engineered cohesin loading module can be created by fusing the Scc2 C terminus to RSC or to other chromatin remodelers, but not to unrelated DNA binding proteins. These observations demonstrate the importance of nucleosome-free DNA for cohesin loading and provide insight into how cohesin accesses DNA during its varied chromosomal activities. Despite our increasing understanding of cohesin, how this essential protein complex accesses chromosomes is incompletely understood. Muñoz et al. provide insight into this by revealing that a molecular machine that mobilizes nucleosomes assists with cohesin loading and defines the DNA entry points for cohesin in the context of chromatin.
Muñoz, S., Minamino, M., Casas-Delucchi, C. S., Patel, H., & Uhlmann, F. (2019). A Role for Chromatin Remodeling in Cohesin Loading onto Chromosomes. Molecular Cell, 74(4), 664-673.e5. https://doi.org/10.1016/j.molcel.2019.02.027