Kinetics of interaction between ADP-ribosylation factor-1 (Arf1) and the Sec7 domain of arno guanine nucleotide exchange factor, modulation by allosteric factors, and the uncompetitive inhibitor brefeldin A

Abstract

Background: Kinetic modulations of Arf1-Sec7 domain complex, by the uncompetitive inhibitor brefeldin A and allosteric factors, are not established. Results: Brefeldin A reorients the binary Arf1-Sec7 domain complex to an abortive one with reduced association and dissociation rates. Conclusion: Kinetic hallmarks allow distinguishing the level, nature, and fate of interacting species. Significance: Similar approach will solve the inhibitory mechanism of new inhibitor families of sec7 domains.© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.