The effects of soxhlet and power ultrasonic-assisted soxhlet extraction on the yield of terpenoids extract from Jasminum sambac

Abstract

This work is about the effect of two extraction methods namely Soxhlet extraction and power ultrasonic-assisted Soxhlet extraction (PUASE) to extract terpenoids from Jasminum sambac. The extraction parameters on yield of terpenoids for Soxhlet extraction are solvent composition (25%, 50%, 75% and 100% methanol) and extraction time (6, 9 and 12 h), while for PUASE, the parameter are ultrasonic amplitude (25%, 50% and 75%; 700W, 20kHz) and exposure time (15, 30 and 45 min), respectively. The solid-to-solvent ratio is set to 1:10. The overall time used in PUASE is set according to the time of Soxhlet extraction that produced maximum yield of terpenoids which is 12 hours and Power ultrasonic were used as a pre-treatment. The extracted essential oil containing terpenoids is qualified by Fourier-Transform infrared spectroscopy (FTIR) and quantified by Ultraviolet-Visible spectroscopy (UV-Vis) at 608nm wavelength via vanilin-H2SO4 method. PUASE shows increase yield of terpenoids produced up to 63% compared to Soxhlet extraction with 15 minutes of pre-treatment. This is suggested due to initial pre-treatment with power ultrasonic causes rupture to cell wall structure which explain the increased amount of terpenoids extracted. In this regards, the effect of both Soxhlet extraction and PUASE for extracting terpenoids is discussed.