Alzheimer’s disease, the most common neurodegenerative disease, is characterized by a progressive loss of synapses and accumulation of amyloid-beta (Aβ) peptides in the brain. Previous studies demonstrated that acute increase in synaptic activity in cultured hippocampal slices and mouse brains (Cirrito et al. Neuron 48: 913–922, 2005; Kamenetz et al. Neuron 37: 925–937, 2003) enhanced secretion of Aβ. Since synaptic activity promotes Aβ secretion, it could also affect the trafficking and processing of its precursor, the amyloid precursor protein (APP). Here, we describe a method to investigate the effect of acute synaptic activation on APP trafficking within dendrites.
CITATION STYLE
Tampellini, D., & Gouras, G. K. (2011). Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging. In Methods in Molecular Biology (Vol. 793, pp. 343–350). Humana Press Inc. https://doi.org/10.1007/978-1-61779-328-8_22
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