Flavobacterium fluvii sp. nov., isolated from stream sediment

Abstract

An aerobic, yellow-pigmented, Gram-staining-negative bacterium, designated strain H7T, was isolated from sediment of Gazwa stream in Jinju city, South Korea. Cells of strain H7T were nonmotile, straight rods that produced flexirubin pigments and showed catalase- and oxidase activities. Strain H7T contained iso-C15:0, anteiso-C15:0 and summed feature 3 (comprising C16:1ω7c and/or iso-C1:5:0 2-OH) as the main fatty acids and menaquinone-6 (MK-6) as the major isoprenoid quinone. The DNA G+C content of strain H7T was 37.2 mol%. Comparative 16S rRNA gene sequence analysis showed that strain H7T formed a distinct phyletic line within the genus Flavobacterium. Based on 16S rRNA gene sequence similarities, the novel strain was most closely related to Flavobacterium limicola ST-82T and Flavobacterium resistens BD-b365T with 97.2% and 97.0% gene sequence similarities, respectively. The DNA-DNA relatedness of the novel strain with these species was only 18% and 14 %, respectively. On the basis of phenotypic data and molecular properties, strain H7T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium fluvii sp. nov. is proposed. The type strain is H7T (=KACC 12818T=DSM 19978T). © 2010 IUMS.