Detection of Coronavirus Infection of Man by Immunofluorescence

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Abstract

An indirect fluorescent antibody technique has been developed for use with coronaviruses 229E and OC43. For the former virus, WI-38 cells were used as the host system, and for the latter LLC-MK2 cells were employed. Human serum pairs which were known to show a rise in antibody titer by other serologic techniques were tested by the FA method. Comparison of results obtained by different procedures showed that FA was specific and would be a useful addition to available methods for identifying infection serologically. © 1977, SAGE Publications. All rights reserved.

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Monto, A. S., & Rhodes, L. M. (1977). Detection of Coronavirus Infection of Man by Immunofluorescence. Proceedings of the Society for Experimental Biology and Medicine, 155(2), 143–148. https://doi.org/10.3181/00379727-155-39761

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