Rapid typing of herpes simplex virus isolates by deoxyribonucleic acid: Deoxyribonucleic acid hybridization

Abstract

A method for typing clinical isolates of herpes simplex virus was developed. It utilizes hybridization between unlabeled deoyribonucleic acid from infected cultures and tritium-labeled virus deoxyribonucleic acid, and it can be completed within a day using a single roller-tube culture of the clinical isolate. The data obtained are inherently quantitative, and the method yields unequivocal identification and typing. Thirty-nine coded clinical isolates were all correctly typed by this method.